Growth and Production Optimization of Tropane Alkaloids in Datura stramonium Cell Suspension Culture

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GC-MS investigation of tropane alkaloids in Datura stramonium.

Alkaloids, GS-MS, Datura stramonium The alkaloid spectrum in roots, leaves and seeds of Datura stramonium L. was investigated by GC-MS. Twenty-nine tropane alkaloids are detected. Twelve of them are new constituents for the species and the two tropane esters 3-(3'-acetoxytropoyloxy)tropane (21) and 3-(2'-hydroxytropoyloxy)tropane (26) are described for the first time.

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An analysis of Sorghum halepense’s behavior in presence of tropane alkaloids from Datura stramonium extracts

BACKGROUND This study aimed to quantify the allelopathic potential of Datura stramonium (Jimson weed). Sorghum halepense (Johnsongrass) tolerance was assessed by germinating, seed and growing seedlings, dosing of photo-synthesis pigments, followed by treatment with D. stramonium extract tropane alkaloids. RESULTS Preliminary chemical analysis of the extracts showed the presence of alkaloids.T...

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Tropane alkaloids of Datura innoxia from Morocco.

Fifty three alkaloids were identified in the organs (roots, stems, leaves, flowers, and seeds) of Datura innoxia by GC/MS. Seventeen of them are reported for the first time for this species and one nor-derivative, 3-phenylacetoxynortropane (28), for the genus Datura. Furthermore, four new tropane esters were tentatively identified as 3-acetoxy-6,7-epoxytropane (acetylscopine) (10), 3-acetoxy-6-...

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Growth and Production of Tropane Alkaloids by Cell Suspension Cutures of Hyoscyamus Albus

Cell suspension of Hyoscyamus a/bus L. were investigated for growth and production of tropane alkaloids in three different media. The effects of sucrose concentration and initial pH were also studied. 2,4Dichlorophenoxyacetic acid and 1% sucrose enhanced the production of tropane alkaloids. The percent of alkaloids (0.75%) was higher than in the cultivated plant (0.352 %).

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Effect of Sodium Chloride on Tropane Alkaloids Accumulation and Proline content in Datura metel and D. stramonium callus cultures.

Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura species. However, MS medium supplemented with 1mg/l of both BA and NAA was the best for callus production in D. metel, while MS supplemented wit...

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ژورنال

عنوان ژورنال: Pakistan Journal of Biological Sciences

سال: 2007

ISSN: 1028-8880

DOI: 10.3923/pjbs.2007.1236.1242